An easy procedure to transform the ratio of two polynomials of first degree into Michaelis-Menten-type equations. Application to the ordered Uni Bi enzyme mechanism.

نویسندگان

  • R Fontes
  • J M Ribeiro
  • A Sillero
چکیده

It is not always clear that some equations affected by complicated factors can, actually, be interpreted as a ratio of two polynomials of first degree and so that they can be, in general, represented by rectangular hyperbolas. In this paper we present an easy procedure to rearrange those equations into Michaelis-Menten-type equations and so to make the aspects of these rectangular hyperbolas more clear, particularly for researchers familiar with general biochemistry. As an example, the method is applied to transform the classical rate equation of the Cleland's Ordered Uni Bi enzyme mechanism.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Analytical Solution of Steady State Substrate Concentration of an Immobilized Enzyme Kinetics by Laplace Transform Homotopy Perturbation Method

The nonlinear dynamical system modeling the immobilized enzyme kinetics with Michaelis-Menten mechanism for an irreversible reaction without external mass transfer resistance is considered. Laplace transform homotopy perturbation method is used to obtain the approximate solution of the governing nonlinear differential equation, which consists in determining the series solution convergent to the...

متن کامل

Kinetics and Mechanism of Oxidation of n-Pentanol by Tetramethylammonium Fluorochromate

The oxidation of n-pentanol by tetramethylammonium fluorochromate in acidic solution wasstudied using spectrophotometric technique. The reaction was arranged to be under pseudo firstorderconditions respect to the oxidant. A Michaelis-Menten type kinetic was observed respect tothe substrate. The reaction is catalyzed by hydrogen ions. Dependences of the reaction rates ontemperature and different...

متن کامل

Biochemical Characterization of A Novel Thermophilic Esterase Isolated from Shewanella sp F88

The main objective of this study was to purify and characterize an esterase from Shewanella sp F88. The enzyme was purified 41-fold and an overall yield of 21 %, using a two-step procedure, including ammonium sulfate precipitation and Q-sepharore chromatography. Molecular weight of the enzyme was 62.3 kDa according to SDS-PAGE data. The enzyme showed an optimum activity at pH 6.5 and 58 ˚C. Evo...

متن کامل

Application of Laguerre Polynomials for Solving Infinite Boundary Integro-Differential Equations

In this study‎, ‎an efficient method is presented for solving infinite boundary integro-differential equations (IBI-DE) of the second kind with degenerate kernel in terms of Laguerre polynomials‎. ‎Properties of these polynomials and operational matrix of integration are first presented‎. ‎These properties are then used to transform the integral equation to a matrix equation which corresponds t...

متن کامل

Application of Chebyshev Polynomials for Solving Abel's Integral Equations of the First and Second Kind

In this paper, a numerical implementation of an expansion method is developed for solving Abel's integral equations of the first and second kind. The solution of such equations may demonstrate a singular behaviour in the neighbourhood of the initial point of the interval ofintegration. The suggested method is based on the use of Taylor series expansion to overcome the singularity which le...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Acta biochimica Polonica

دوره 47 1  شماره 

صفحات  -

تاریخ انتشار 2000